ABSTRACT
OBJECTIVE: The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. MATERIAL AND METHODS: Undiluted (crude extract) and diluted extracts to 10 percent, 1 percent, 0.1 percent, 0.01 percent, 0.001 percent and 0.0001 percent of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5 percent significance level. RESULTS: Crude extract of AH Plus and Fill Canal killed approximately 90 percent of THP-1 cells versus 36 percent of THP-1 cells killed by L&C crude extract (p<0.05). Ten-fold dilutions of L&C, Fill Canal and AH Plus killed 24, 35 and 61 percent of THP-1 cells (p<0.05), respectively. Dilutions lesser than 1 percent caused minimal cell death as compared to the control groups (p>0.05), except for L&C 1 percent extract. CONCLUSIONS: The results revealed that the L&C paste crude extract was less cytotoxic to THP-1 cells than AH Plus or Fill Canal crude extracts.
Subject(s)
Humans , Calcium Hydroxide/toxicity , Root Canal Filling Materials/toxicity , Barium Sulfate/toxicity , Bismuth/toxicity , Borates/toxicity , Cell Count , Cell Line, Tumor , Cell Death/drug effects , Cell Survival/drug effects , Coloring Agents , Drug Combinations , Epoxy Resins/toxicity , Eugenol/toxicity , Materials Testing , Monocytes/drug effects , Resins, Synthetic/toxicity , Trypan Blue , Zinc Oxide-Eugenol Cement/toxicity , Zinc Oxide/toxicityABSTRACT
Se describe 14 pacientes intoxicados por ingesta inadvertida de sales solubles de Bario (sulfito y sulfuro). Fueron 11 varones y 3 mujeres, con un promedio de edad de 37 años. La sintomatología predominante estuvo dada por molestias gastrointestinales (diarrea, náusea, vómito y dolor abdominal) y neuromusculares (mareo, debilidad, parestesia y dificultad respiratoria); que iniciaron a las pocas horas de la ingesta. Hubo una correlación estrecha entre la sintomatología neuromuscular y la deplesión de potasio sérico (valor promedio: 2.72 mEq/L); tanbién se observó deplesión de magnesio sérico en 5 pacientes (valor promedio: 1.16 mEq/L), relacionándose a la existencia de paresia muscular. El estudio electromiográfico en 2 pacientes encontró alteración miopática definida. El tratamiento estuvo dirigido al disturbio electrolítico de potasio y magnesio. Un paciente falleció en paro cardio-respiratorio y el resto se recuperó ad-integrum en 24 a 36 Hrs